The degree of substitution of carboxymethylcellulose (CMC) can be determined by various methods, one of which is commonly used by infrared spectroscopy.
Infrared spectroscopy is a technique based on the absorption of infrared radiation by substances and can be used to detect and analyze the structure and composition of chemical substances. When determining the degree of substitution of carboxymethyl cellulose (CMC), an infrared spectrometer can be used to measure the absorption lines of the sample in a certain wavelength range, and then compare it with the reference standard.
Specifically, the degree of substitution can be calculated by measuring the absorption peaks of hydroxyl (OH) and carboxyl (COOH) groups in carboxymethylcellulose (CMC) molecules. Generally speaking, the higher the degree of substitution of carboxymethylcellulose (CMC), the weaker the OH peak and the stronger the COOH peak.
In addition, there are some other methods to determine the degree of substitution of carboxymethyl cellulose (CMC), such as effective liquid chromatography (HPLC), nuclear magnetic resonance spectroscopy (NMR), etc. These methods have their own characteristics, and it is necessary to choose an appropriate method for determination according to the actual situation.
