To determine the hexenuronic acid content of pulp by UV spectroscopy, the following procedure can be used:
Sample preparation: Take a certain amount of pulp sample and dilute it with water to an appropriate concentration.
Preparation of standard curve: Prepare standard solutions of hexenuronic acid with different concentrations, measure the absorbance according to the absorption spectrum, and make a standard curve.
Determination of sample absorbance: Use an ultraviolet Spectrophotometer to absorb the pulp sample and record its absorbance.
Calculation of content: According to the standard curve, convert the absorbance of the obtained sample into the concentration of hexenuronic acid, and then calculate its content in the pulp according to the dilution factor of the sample taken.
NBCHAO reminds you: Before the measurement, the instrument should be preheated and zero-point adjusted, and the interference of other impurities should be avoided. If a more accurate measurement is required, multiple experiments can be carried out and the average value taken.
