Spectrophotometric methods can be used to determine the content of pentosan in raw materials and pulp. The following are the measurement steps:
Sample preparation: Take an appropriate amount of the sample to be tested and add it into distilled water to prepare a solution with a certain concentration.
Spectrophotometric measurement: put the prepared solution into a Spectrophotometer , set the wavelength to 620nm for measurement.
Preparation of standard curve: Prepare a series of standard solutions of pentosan with different concentrations, and use the same method for determination. The absorbance values and concentrations of standard solutions with different concentrations were plotted into a standard curve.
Calculate the content of pentosan in the sample to be tested: use the absorbance value of the sample to be tested to find the corresponding concentration on the standard curve, multiply it by the dilution factor, and then calculate the content of pentosan in the sample.
NBCHAO reminds you: the accuracy and precision of spectrophotometry are affected by many factors, such as sample preparation, instrument adjustment, optical path calibration, standard curve preparation, etc. Therefore, it is necessary to operate carefully during the measurement and strictly control the errors in each link.
