O-quinones and conjugated carbonyl groups are one of the main components of lignin, which can be quantitatively determined by spectrophotometry. Specific steps are as follows:
Preparation of standard curve: Take a certain concentration of o-quinone or conjugated carbonyl standard, and dilute them into standard solutions with a series of concentrations.
Determination of sample absorbance: Take a certain amount of sample, convert it into o-quinone or conjugated carbonyl, and then use a Spectrophotometer to measure its absorbance.
Calculation of content: Substitute the absorbance value of the sample into the standard curve to calculate the content of o-quinone or conjugated carbonyl in the sample.
Beichao reminds you: when converting samples to o-quinones or conjugated carbonyls, sodium hydroxide or a mixture of sodium hydroxide and sulfuric acid can be used as a catalyst. At the same time, when measuring the absorbance of a sample, it is necessary to select an appropriate measurement wavelength according to the actual situation.
