Neutral xylanase (Endo-β-1,4-xylanase) is an enzyme that hydrolyzes xylan, and its activity can be determined by the following methods:
Turbidimetric method: take xylan as the substrate, add an appropriate amount of neutral xylanase preparation, react within a certain period of time, then add phenolphthalein indicator, titrate with NaOH to the color change point, and record the titer. The activity of neutral xylanase was calculated according to the change of optical density of the reaction solution.
Phenol-sulfuric acid method: take xylan as a substrate, add an appropriate amount of neutral xylanase preparation, react within a certain period of time, then add phenol reagent and sulfuric acid to produce a color change, measure the absorbance with a Spectrophotometer , and compare with The activity of neutral xylanase was calculated according to the standard curve.
HPLC method: take xylan as a substrate, add an appropriate amount of neutral xylanase preparation, react within a certain period of time, and then use an effective liquid chromatography (HPLC) to separate and detect the reaction product, by peak area or peak High calculated neutral xylanase activity.
NBCHAO reminds you: When measuring the activity of neutral xylanase, the reaction conditions should be controlled, such as reaction temperature, pH, reaction time, etc., to ensure the accuracy and repeatability of the experimental results.
