Alkaline xylanase is an enzyme preparation, and its activity can be determined by the following steps:
Sample preparation: Dissolve the alkaline xylanase preparation and prepare an enzyme solution with a certain concentration, and adjust the pH to an appropriate range with a buffer.
Prepare the substrate: select a substrate suitable for the enzyme, such as alkaline xylan, and prepare a substrate solution with a certain concentration.
Reaction conditions: Add a certain amount of enzyme solution into a certain amount of substrate solution at a suitable temperature and pH, and react for a certain period of time.
Termination of the reaction: the reaction is terminated by adding a suitable acid or base, and the reaction solution is in a suitable pH range.
Determination of products: measure the concentration of the products produced by colorimetry, fluorescence or effective liquid chromatography, and calculate the activity of the enzyme.
NBCHAO reminds you: When measuring activity, it is necessary to control the reaction conditions, ensure that the concentration of substrate and enzyme is appropriate, the reaction time is appropriate, the termination of the reaction is accurate, and the appropriate determination method and standard curve should be selected.
