Microplates are used for internal verification or calibration of microplate readers. Use them to calibrate the wavelength scale and photometric scale of the device.
Microplates are typically rectangular, approximately 12.5 cm x 8.5 cm in size, and consist of 8 rows by 12 columns, containing 96 wells. Each of these 12 columns represents a specific absorbance value at a different wavelength, and in some cases there are columns for wavelength scale calibration containing holmium oxide filters with known absorbance peaks.
Absorbance Calibration
Absorbance calibration, or the calibration of a photometric scale, allows the accuracy or correction of a measurement device relative to the absorbance values presented at different wavelengths.
Microplates consist of 5 or 6 neutral density filters with different absorbance values arranged along columns. These filters exhibit absorbance values ranging from 0.2 OD to 3.5 OD.
wavelength calibration
There are some models of microplates that include columns for calibration of wavelength scales. With this type of calibration, the ability of the Spectrophotometer 's monochromator component to separate specific wavelengths from the rest of the illumination spectrum can be evaluated.
Microplates with this particularity contain filters in their columns with narrow absorption bands for different wavelengths. These filters are usually made of holmium oxide and have absorption peaks in the UV-visible range from 240nm to 650nm.
