Pectin is a polysaccharide widely present in plant cell walls, and its content can be determined by phenol-sulfuric acid method or effective liquid chromatography.
The steps of the phenol-sulfuric acid method are as follows:
Sample preparation: The samples were pulverized and screened, and an appropriate amount of samples were taken and weighed.
Extraction: Add a certain amount of hot water (usually 80°C) to the sample, heat it for a certain period of time, and then filter it.
Precipitation: add a certain amount of alcohol to the extracted liquid, collect the precipitate after precipitation and dry it.
Dissolution: Dissolve the precipitate in a phenol-sulfuric acid mixture (usually a mixture of 75% phenol and concentrated sulfuric acid), and heat for a certain period of time to completely dissolve the sample.
Colorimetry: Cool the dissolved sample to room temperature, and measure the absorbance using a UV-Vis Spectrophotometer .
Calculation: Calculate the pectin content according to the absorption characteristics of the phenol-sulfuric acid reagent and the absorbance of the control sample.
The effective liquid chromatography steps are as follows:
Sample preparation: The samples were pulverized and screened, and an appropriate amount of samples were taken and weighed.
Extraction: Add the sample to an appropriate amount of solvent for extraction (usually 80% methanol or ethanol), heat for a certain period of time, and then filter.
Purification: Passing the extracted fluid through certain purification steps, such as ion exchange chromatography or gel chromatography, to remove interfering substances.
Analysis: inject the sample liquid into an effective liquid chromatograph for analysis, and detect the content of pectin.
NBCHAO reminds you: In actual operation, it is necessary to strictly control the sample preparation and operation process, as well as the adjustment of instrument conditions, in order to ensure the accuracy and reliability of the experimental results.
