Hydroxyl content usually refers to the content of hydroxyl (OH) in organic compounds, and its measurement method varies with different substances. The following are two common methods of determination:
Carboxymethyl cellulose assay:
Add the sample to be tested into sodium bicarbonate buffer (pH=10.5), add hydroxylamine hydrochloride, heat the reaction in a boiling Water Bath for a certain period of time, stop the reaction, and add isopropanol to precipitate it. After the precipitate was washed, filtered and dried, the hydroxyl content in it was determined by the phenol-sulfuric acid colorimetric method.
UV absorption spectrometry:
Dissolve the analyte in water, measure its absorbance at 220 nm with an ultraviolet spectrometer, and then calculate the hydroxyl content in it according to the intensity and slope of the absorption peak. This method is suitable for macromolecular organic compounds containing hydroxyl groups, such as polysaccharides, plant extracts, etc.
Beichao reminds you: Different compounds and samples may require different hydroxyl determination methods, and different methods may also have differences in the operation process and measurement results. Therefore, when selecting a method for the determination of hydroxyl content, it is necessary to select an appropriate method according to the characteristics of the substance to be tested and the required accuracy.
