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This manual is compiled based on manufacturer's data. Due to equipment batch changes, the content may not fully match every physical unit. Please refer to the actual operation and on-device labels.

Copyright belongs to the manufacturer. For internal reference of purchasers only. If safety parameters in the manual differ from the physical unit, please suspend operation and consult our engineer for confirmation.

Disclaimer: This manual is for reference only. In case of any discrepancy, the physical unit shall prevail. Our company assumes no liability for any consequences arising from operations based on this manual.

YIHENG BPC-F with printed biochemistry cultivating box instruction manual

YIHENG BPC-F with printed biochemistry cultivating box manual explains temperature control operation, timing setting, over-temperature alarm, optional printer use and troubleshooting, helping users master the functions of incubator.

Safety tips

Danger (may result in serious property loss or personal injury)

  1. This product must be reliably grounded and kept away from sources of electromagnetic interference (neutral or centerline must not be used as ground).

  2. Before use, please confirm that the voltage and frequency of the power supply match the product requirements.

  3. Products should use independent power outlets, and ensure that plugs and sockets are properly grounded.

  4. It is not allowed to unplug or plug in the power cord arbitrarily without turning off the power switch during operation.

  5. It is not allowed to arbitrarily extend or cut the product's power cord.

  6. Repairs must not be performed without authorization; repairs commissioned by our company must be performed by professionals.

Warning (may result in property damage or personal injury)

  1. You must fully read and understand the product manual before proceeding.

  2. The 304 stainless steel inner tank is not acid-resistant. Please take anti-corrosion measures, and never use acidic media inside the box!

  3. When unplugging the power cord, do not drag the power cord directly.

  4. If any of the following situations occur, you must unplug the power cord of this product:

    1. When replacing the fuse;

    2. When a product malfunction is awaiting inspection and repair;

    3. When the product is discontinued for a long time;

    4. When moving products.

Note (may affect service life and cause the product to malfunction)

  1. During product handling, the tilt angle must not exceed 45 degrees to avoid damage to the refrigeration system.

  2. After the product is transported and placed in place, it should be left to stand for (1~2) days before starting the machine, to ensure the refrigeration system operates normally and extends its lifespan.

  3. Products should be placed on a hard and solid surface to keep them level.

  4. A certain amount of space should be left around the product.

  5. Products must be used under certain operating conditions.

  6. Do not forcefully open or close the product box door, as this may cause the door to fall off, damage the product, and lead to injury accidents.

  7. When the product is out of use for a long time, regular moisture removal should be performed to avoid damaging related components.

Product Introduction

1. Appearance drawing

External diagram of the biochemical incubator        

BPC series biochemical incubators

(1) Controller (2) Box door (3) Observation window (4) Door handle (5) Wheel feet (6) Microprinter (optional) (7) Test port (8) UV lamp switch (9) Refrigeration transfer switch (10) Power switch (11) Independent temperature limiting controller (optional)

2. Overview of structural functions

Cabinet: The casing is stamped from steel plates with a surface spray coating; The inner liner is made of mirror-finish stainless steel, with semi-circular curved corners that are very easy to clean, and the spacing between shelves inside the box is adjustable.

Cycle: Inside the enclosure, there are cold and hot air flow ducts, and the fan operation enhances smooth gas circulation to improve the uniformity of the studio temperature.

Control: A digital circuit controller composed of a CPU-programmable processor, featuring PID adjustment characteristics, timing control, error correction, and deviation alarm functions. Together with a Pt100 platinum resistor and heating (cooling) component, it forms a closed-loop automatic control system.

Display: It uses an LCD black screen with a user-friendly display of text and images.

Protection: Equipped with delayed compressor start and overheat protection functions; Equipped with a multi-level independent over-temperature alarm protection system:

  • Level 1 Over-Temperature Protection: The temperature controller is equipped with an upper deviation alarm function, allowing users to adjust the AL1 deviation parameter as needed.

  • Level 2 Over-Temperature Protection: When the actual temperature of the equipment exceeds the high-temperature limit set protection value, the heating section is automatically cut off and an audible and visual alarm is triggered. Since the circulation fan is still operating normally during the alarm, the alarm will automatically be canceled when the temperature falls below the set value. (User optional part)

  • Level 3 Over-Temperature Protection: The electric heating pipe circuit is connected in series with an overheat protector. When the temperature inside the box reaches the maximum temperature control value, the temperature relay automatically disconnects to prevent dangerous situations. When the temperature falls below the control value, it returns to normal.

Convenience: The biochemical box door has a large double-glazed observation window. On the right side of the enclosure, there is a measuring hole with a diameter of φ25mm, which facilitates temperature measurement.

Environmental protection: The refrigeration system uses fluorine-free refrigerants to meet environmental protection requirements.

Functional Scalability: Supports RS232/RS485 interfaces for remote control; (Optional) Optional micro printer for real-time printing and long-term data preservation; (Optional)

Product usage

1. Preparation before use

Products should operate under the following usage conditions:

  1. Ambient temperature: 15°C~35°C, relative humidity not exceeding 85%;

  2. Power supply: (220±22)V (50±1)Hz;

  3. Atmospheric pressure: (86~106) Kpa;

Usage conditions diagram        

Figure 2

  1. No strong vibration sources or strong electromagnetic fields nearby;

  2. It should be placed in a stable, level indoor area free of heavy dust, direct sunlight, and corrosive gases;

  3. Leave sufficient space around the product, as shown in Figure 2;

  4. Arrange them properly, adjust the position and quantity of shelves, and when placing items in the studio, ensure there are sufficient gaps around the top and bottom, and the weight should be so that the shelves are not bent or deformed by pressure.

2. Description of the thermometer panel

Thermometer panel diagram        Thermometer panel diagram        

Figure 3: Operation and temperature controller panel diagram

1.1 Indicator Light Description

  • TEMP Zone (PV): Displays measurement temperature;

  • SET Zone (SV): Displays the set temperature;

  • Heating lamp: The light turns on when there is heating output;

  • Cooling Lights: Delayed flashing, lights up when there is cooling output; Turns off when there is no cooling output;

  • Fan indicator light: Lights up when the fan is outputting;

  • Printing Functions: Lights up when printing is present; (Optional)

  • TIME: Time display window; Displays runtime or parameter values.

1.2 Key Description

  • Shift key: Used for shifting set values, internal parameters, and viewing ambient temperature;

  • Decrease key: Used for modifying set values, various parameters, or start/stop auto-tuning;

  • OK key: Used to modify set values or enter internal parameter settings; press and hold the setting button for more than 3 seconds while parameter setting mode to exit;

  • Key: Press for 3 seconds to run or stop the controller;

  • Add key: Used for setting values, internal parameters, or checking remaining cycles.

2. Operating steps

  1. Timer Function: Press the "OK" button once; when the time is set to 0, the timer function is disabled; When the time setting is not zero, the controller has a timer function. Press the "OK" button, and the TIME value flashes, indicating the time can be set as needed. Use the "Increase", "Decrease", and "Shift" keys to set the desired time value. When the timer is up, the TIME window displays "END" and the buzzer sounds. You can mute it with any key.

  2. Temperature settings: Press the "OK" button again; the SET window value will flash, indicating the temperature can be set as needed. Use the "Increase", "Decrease", and "Shift" keys to set the desired temperature. Press the "OK" button again to return to standard display mode.

Note: (1) For each parameter modification, press the "OK" key to confirm for the modification to be valid.

(2) After setting all parameters, press the " " key and wait about 4 seconds before starting operation.

3. Setting up the upper deviation alarm

The setting of the upper deviation is reasonable, providing protection against system temperature control exceeding or losing control, and must be used during product operation.

Example: If the product is set to AL=50 at the factory, the alarm temperature is: (set temperature value + AL value)°C

4. Check the accuracy of temperature control

To ensure successful cultivation, use it during the first use or at regular intervals.

  1. Place a 0.5°C grading mercury thermometer (or a digital thermometer with 0.1°C resolution) into the product workroom;

The mercury thermometer head should be positioned at the geometric center of the effective workspace in the studio

  1. Within the product's temperature control range, select any point and set the SV temperature control value. When the PV measurement equals the set value, maintain the temperature for about 1~2 hours (the duration varies depending on product specifications). Observe that the actual temperature measured by the mercury thermometer and the PV readings displayed by the thermometer should be less than or equal to the temperature control fluctuation.

  2. After the product has been used for a period of time, the temperature control accuracy should be checked according to the method in 4.2. If it exceeds ±1°C, the following corrections can be made:

4.3.1 Enter the thermostat C menu;

4.3.2 Press the function key several more times to find the L66 symbol,

According to PK = 4000 × (Measured value PV - mercury meter reading) / mercury meter reading

After formula calculation, use keys to modify the original factory PK value (Note: If one correction is inaccurate, it can be revised repeatedly until it matches).

5. How to use the optional "independent temperature limiting controller"

An independent temperature limiting controller is a separate protection system. When the temperature controller malfunctions and causes temperature loss and the temperature inside the workroom reaches the temperature limit set on the over-temperature dial, the independent temperature controller will automatically cut off heating and sound an alarm.

(As shown in the figure on the right) When the temperature inside the workroom falls below the temperature limit set value, the protection system is deactivated and the instrument resumes operation.

This cycle repeats until the fault is resolved.

The specific steps are as follows:

  1. The temperature limit set value should be greater than or equal to (SV + AL) + (2~5)°C

Independent temperature limiting controller    
  1. See Figure 4. Use the + and — buttons on the over-temperature setting dial on the panel to set the required temperature limit.

Example: SV=37°C, AL=2, then 42°C should be set (i.e., dial set 042).

Set button        

Set button

Figure 4

Product maintenance and precautions

1. Product storage conditions

  1. Ambient temperature: (-40~+55°C)

  2. Relative humidity: ≤95% (25°C)

  3. Atmospheric pressure: (50~106) Kpa

2. Methods to remove moisture

  1. If the equipment will not be used for a long time, unplug the power cord to prevent injury;

  2. Regularly (usually in the first quarter), power on and heat up according to usage conditions for 5 hours to remove moisture from electrical components and avoid damaging related components;

  3. Set the temperature to 40°C and open the door every two hours to release moisture;

  4. After processing, unplug the power cord and dry the moisture inside the box for storage.

3. Defrost treatment

  1. When the equipment operates below 5°C or at temperatures below ambient temperature for extended periods;

  2. When cooling performance is not ideal (slow cooling or static error);

  3. According to Clause 3.1, regular "defrosting" should be performed: set to 40°C and let the equipment operate for more than 3 hours;

  4. After defrosting, reset the required parameters and start operation.

  1. After use, turn off the main circuit power and wipe off the moisture inside the chamber.

  2. Before reuse or when process requirements change, temperature control accuracy verification should be conducted. (See relevant sections)

  3. Except for parameters such as TIME and SET, other control parameters require approval from our service center or are adjusted by professionals.

Appendix

1. Technical Specifications

This product is manufactured according to the company standard Q/TIWY 11

Table 1

Serial numberModel specificationsBPC-70FBPC-150FBPC-250F
1Power supply(220±22) V (50±1)Hz
2Temperature control range0°C ~ 60°C
3Temperature resolution0.1°C
4Temperature fluctuation±0.5°C
5Inner Pot Dimensions (mm)400×450×500500×470×800520×560×1050
6Dimensions (mm)510×530×970650×620×1350670×740×1590
7Cargo shelvesTwo piecesThree piecesThree pieces
8RefrigerantsR134a
9Input Power (W)500700850

2. Functional parameter tables

1) Menu B: Press and hold the OK key. When the LCD shows LK, enter the password and press OK to enter the settings;

Table 2

PromptNameSet the rangeExplanationFactory value
KAPower-on mode0~31 When KA=0, the controller is in a stopped state after powering on, and must be started and running by long-pressing the start/stop button; 2 When KA=1, the controller automatically starts after powering on; 3 When KA=2, the controller starts operating from the last power cut-off point
SEAmbient temperature correction-19.9-80.0If the ambient temperature is inaccurate from the actual ambient temperature, correct the value to match it./
PbZero position adjustment-100.0~100.0When the controller's zero error is large and the fullness error is small, adjust this value; generally, Pt100 rarely adjusts this value.
PkFull level adjustment-1000~1000 secondsWhen the controller's zero error is small and the fullness error is large, this value can be adjusted.

2) Menu C: Press and hold the OK key. When the LCD screen shows LK, enter the password and press OK to enter the settings;

Table 3

PromptNameSet the rangeExplanationFactory value
ALAlarm settings0~100.0°CWhen the measured temperature exceeds the "set temperature + AL" value, the over-temperature alarm indicator lights up and cuts off the heating output
CLRefrigeration control settings0.0~100.0When the temperature exceeds the "set temperature + CL" and meets the compressor cooling delay time, the refrigeration indicator light turns on, the refrigeration contacts are connected, and the compressor is started
CtCooling control delay0~3600 (seconds)The delay time required for two consecutive compressor starts is Ct=0 to cancel the compressor function
PScale band1.0~300.0Proportional action adjustment: the larger the P, the smaller the proportional effect, the lower the system gain, acting only on the heating side.
IScoring time10~3600SThe time constant for the effect of integrals, the larger I, the weaker the integral.
dDifferential time0~3600SThe time constant of differential action: the larger d, the stronger the differential action, and it can overcome overshoot (D=0 PI control)/
ArOvershoot suppression0~100%Used to suppress overshoot (Ar is determined as: 1.5~2 times steady-state output duty cycle)
tControl cycle1~300SThe thyristor output is generally 2~3 seconds. For devices with large remaining power, increasing T can reduce the static difference of PID control.

※ Products undergo strict testing before leaving the factory. When technical specifications meet requirements and work normally, generally no corrections are required.

3. Printer Instructions (Optional)

  1. Wiring diagram and indicator light description:

Printer wiring diagram    

Wiring instructions:

Terminals 1 and 2 are communication interfaces (connected to the instrument's communication port), terminals 5 and 6 connect to a 220V power supply, 7 is the debugging interface, and 8 is the print test button (click to print test information).

Indicator light description:

D3. Printer online indicator: off means the printer is online; flashing means the printer is faulty or offline.

D2. Communication indicator: On means communication failed; off means communication was successful.

  1. Press and hold the OK key for more than 4 seconds to enter and find the LK code, set LK to 88, then press and hold the "OK" key to enter print parameter settings:

Table 4

MeaningParameter nameExplanation
Print interval time settingPtYLPrint-110/112: 0~240min. If it is 0, cancel printing. YLPrint-111: 0~9999 seconds. If it is 0, cancel printing. To protect printers from printing frequently, if Pt<10 seconds is used, the default is 10 seconds. To change Beijing time, you need to set Pt>60 to Beijing time. After completing the modification, change to the desired print time.
Read the lower limit range of the dataPL0~65°C
Read data with upper range limitsPH0~65°C
YearyrSet the time year for example: 2011 then yr=11, e.g., 2018 then yr=18
monthMHSet the time and month
DaydySet the time and date
hoursHrTime hour setting
minutesMtTime and minute settings
Time is writtenonWhen the modification is complete, make on = 1; Press OK to exit user parameters.

4. Troubleshooting

Table 5

Fault phenomenaSpeculation on the cause of the faultTroubleshooting methods
No power on (indicator light not on)The power outlet has no power or poor contact with the plugRepair
The box's power cord is broken or not properly plugged inRepair and replug
Power switch is broken (or not turned on)Replace or turn on the power switch
The fuse blowsIf the motor burns out after replacement, check whether the motor or other components are short circuited (if the motor is faulty, there may be odors or blackened wires) or the casing (insulation resistance is zero). Repair it before restarting the machine.
The instrument panel shows no displayWiring is detached or instrument panel is burned outReconnect the wires or replace the instruments
The instrument display "☐☐☐☐"Sensor Faulty or Broken Wiring (Detached)Repair or replace Pt100 (100Ω at 0°C, 0.3Ω/°C)
The temperature value exceeds the control rangeUse appropriate temperature values within the specified range
No coolingThe selector switch is in the wrong positionSet the correct position
Compressor failure, pipeline blockage, or R134a leakReplace, remove blockages, and add R134a
Compressor overheat protector activatedWait for the compressor temperature to drop and then naturally recover
Compressor protector burnt out (with a burnt smell)Replace the protector
Temperature loss of controlThe bidirectional thyristor is broken down and has no heated patternReplacement (Model: BTA16)
The thermostat was brokenReplace the thermometer
No Heating (PV<SV)Thermostat broken (no display)Replace the thermostat
There is a heating output graphicSwitch heaters or BTA16 thyristors
No heating output graphicReplace the thermostat
It uses a timer function, where the temperature rises and then fallsSet TIME≠0 to reset the time time
Large temperature control errors or static errorsFan broken (not running)Replace the fan
Pt100 has poor contact, increasing resistanceRewire
The parameter (Pb, Pk) is set incorrectlyReset
Incorrect settings for parameters such as PIDReadjust parameters
Excessive frost in the condenser results in low cooling powerHeating and defrosting (set to 40°C, operating for more than 3 hours)
Abnormal or loud fan noiseThe fan is faulty or the compressor fan is faultyReplace the fan

5. Wiring schematic

Wiring schematic        

(No changes will be noticed)

Packing list

Product Name: Biochemical Incubator

Serial numberCategoryNameUnitQuantityNote
1DocumentsUser manualportion1
2DocumentsPacking listportion1
3DocumentsGuarantee letterportion1
4DocumentsWarranty cardportion1
5Spare partsFuseOnly2
6AccessoriesShelfBlock3Only two BPC-70F units
7Optional accessoriesPrinting paperVolume1