The ISO 21716-1 standard provides general requirements for bioassay methods for the screening of anti-fouling paints in ships and marine technology. The standard aims to evaluate the performance of anti-fouling paints through laboratory biological methods to determine their toxic effects on aquatic organisms and anti-fouling effects. The standard includes detailed provisions on test methods, equipment, experimental conditions, sample preparation, etc., to ensure the effectiveness and environment friendliness of different types of anti-fouling paints in different water environments. This standard provides a scientific basis for the development, quality control and environmental impact assessment of anti-fouling paints, and helps manufacturers and users choose environmentally friendly and effective anti-fouling coatings.