The ISO 8255-1 standard specifies requirements for dimensional tolerances, thickness and Optical inspection characteristics of Microscope Cover Glass. The standard applies to Cover Glass for Microscope observation, ensuring that it has appropriate dimensional accuracy, Optical Properties and Thickness Stability during use. The standard details the dimensional tolerance requirements for Cover Glass, including side length, diagonal error, and tolerance ranges for Glass thickness, while specifying its Optical inspection characteristics to ensure that Cover Glass provides clear, distortion-free observation under Microscope. By following this standard, the manufacturing mass of the cover Glass can be improved, ensuring its Reliability and Accuracy in scientific research and experiments.