GB 18281.3-2000 Specifies biological indicators for heat and humidity sterilization of healthcare products. This standard specifies the characteristics of biological indicators, such as Bacillus thermophilus, and stipulates key indicators such as spore content and moisture and heat resistance. Detail the preparation, Encasement and use of biological indicators. By placing them in heat and humidity sterilization procedures, observe the inactivation of microorganisms, so as to accurately verify the heat and humidity sterilizing effect. This provides reliable monitoring means for the heat and humidity sterilization process of healthcare products, guarantees the sterilized mass, ensures the safety of product use, and effectively prevents and controls the risk of iatrogenic infection.