Steps to Use Model 722 Spectrophotometer

1. Turn on the power, the indicator light is on, the selection switch is set to "T", and the wavelength is adjusted to use the wavelength. The instrument warms up 20 minutes.

2. Open the cover of the sample chamber (the light door is automatically closed), and adjust the "0" knob to make the number display as "00.0". Cover the sample chamber cover, put the cuvette rack in the distilled water calibration position, let the photocell receive light, adjust the transmittance "100%" knob, and make the number display as "100.0".

3. If the display is less than "100.0", you can appropriately increase the number of magnification levels of the micro-current amplifier, but set the magnification to a lower level as much as possible , so that the instrument will have higher stability. But after changing the magnification, you need to re-calibrate "00.0" and "100%" according to step 2 .

4. After preheating, adjust "00.0" and "100%" several times according to step 2, and the instrument can start measuring work.

5. Measurement of absorbance A: adjust the "00.0" and "100%" of the instrument according to step 2, set the selection switch to "A", adjust the absorbance zero adjustment knob, so that the number is displayed as ".000", and then the measured The sample moves into the light path, and the displayed value is the absorbance value of the sample to be measured.

6. Measurement of concentration C: select the switch from "A" to "C", put the sample with calibrated concentration into the optical path, adjust the concentration knob so that the number is displayed as the calibrated value, put the sample to be tested into the optical path, and then Read out the concentration value of the tested sample.

7. If the test wavelength is greatly changed, wait for a while after adjusting "0.00" and "100%" (due to the sharp change of light energy, the photoelectric cell responds slowly after receiving light, and it takes a period of light response balance time), when it is stable, Rescale "00.0" and "100%" to work.

8. The cuvettes matched with each instrument cannot be exchanged individually with the cuvettes on other instruments.


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